Distribution and Dynamic Changes in Matrix Metalloproteinase (MMP)-2, MMP-9, and Collagen in an In Stent Restenosis Process

ObjectiveThe aim of this study was to observe the spatial distribution and dynamic changes matrix metalloproteinase (MMP)-2, MMP-9, collagen in stent restenosis (ISR) explore their influence on ISR.MethodsSixty Z type stents were implanted into common iliac arteries minipigs, which divided 10 groups (six each group) according euthanasia time (6 hours, 1, 3, 7, 14, 28, 56, 84, 168, 336 days). After samples harvested, haematoxylin eosin staining, immunohistochemical Western blotting, Picrosirius red staining performed for all groups.ResultsISR occurred six minipigs 56 day group (percentage diameter stenosis range 71.6%–79.2%, mean ± standard deviation 75.6% 2.5%). The percentage decreased 38.3% 2.7% at days (p < .001). Immunohistochemical showed that MMP-2 MMP-9 strongly stained near internal elastic lamina or damaged parts intima, around struts neointimal lumen surface ISR process. expression significantly lower compared with peaks (seven one [p .001; p = .002], respectively). At days, content reached its maximum (mean integrated optical density 0.73–0.92, 0.82 0.09). From 14 group, mature neointima correlated negatively (γ(36) −0.816; .001) −0.853; During regression period, new positively (γ(24) 0.683; 0.873; .001).ConclusionThis has demonstrated MMP-2, by simulating process from generation after implantation. When occurred, maximum, might contribute ISR. Sixty groups. This